Assessment of Mitochondrial and Nuclear Genes for Molecular Detection during Early Trichinella spiralis Infection
Silvio J Krivokapich, Cinthia L Gonzalez Prous, Graciana M Gatti and Patricia A Arbusti
Article Type: Original Article | First Published: August 31, 2019
Early serological diagnosis of trichinellosis is affected by a long-term immunological "silent" period following infection. This emphasises the need for the development of sensitive diagnostic methods to be used when antibodies cannot be detected. In this study, we assessed the usefulness of three DNA sequences as a direct diagnosis method to detect early infection with Trichinella spiralis in peripheral blood by SYBR green real-time PCR using a murine model. Primers were designed from a nuclear...
Canine and Human Leishmaniasis: Disease Progression to Brazilian Urbanized Areas
Danilo C Miguel and Danilo C Guarnier, DVM
Article Type: Mini Review | First Published: July 17, 2019
Leishmaniasis is a complex disease considered one of the most neglected tropical diseases of the world. The importance and role of domestic dogs and wild canids as a source of leishmaniasis infection is well recognized. The present mini-review discusses a series of epidemiological studies that reinforce the need to understand the role of reservoirs and vectors in the expansion of leishmaniasis to Brazilian urbanized areas, as Brazil is a country of continental dimensions and endemic for leishman...
Use of an Antigen Excreted (Sode) in the Search for Antibodies Anti-Trypanosoma Cruzi in Sera from Pediatric Population of the State of Queretaro, Mexico
Maria Elena Villagran-Herrera, Manuel Sanchez-Moreno, Jose Alejandro Martinez-Ibarra, Ricardo Francisco Mercado-Curiel, Adriana Jheny Rodriguez-Mendez, Javier Avila-Morales, Maria del Carmen Aburto-Fernandez, Nicolas Camacho-Calderon and y Jose Antonio de Diego-Cabrera
Article Type: Research article | First Published: May 10, 2019
The acute phase of Chagas disease can occur at any age, however the pediatric population is usually the most affected (from one to 15 years), so it is very important to detect and treat it as soon as possible to try to eliminate the circulating parasite. The main objective of this research is to present the first seroepidemiological study of the infection by Trypanosoma cruzi, carried out to students of school age of both urban and rural primary schools in the State of Querétaro and at the same...
Plasmodium falciparum Infection among Febrile Patients Attending a Tertiary Healthcare Facility in Central Nigeria: Prevalence, Hematologic and Sociodemographic Factors
Yohanna Jemimah, Oti Victor, Amuta Elizabeth, Philip Akpu and Anizoba Lynda
Article Type: Research article | First Published: April 04, 2019
Plasmodium falciparum infection remains a leading cause of global morbidity and mortality, causing about 3,000 daily deaths. This study intended to document the prevalence, and the associated factors of P. falciparum infection among febrile patients attending Federal the Medical Centre Keffi, Nigeria. After ethical clearance, 400 whole blood samples were collected from patients who gave informed consent and completed a self-structured questionnaire from July 2015 through January 2016. The blood ...