The Blood Levels of the Amino Acid Kynurenine and Indoloxigenase are Predictive of Acute Graft Versus Host Disease (aGVHD)
Ferreira DC, Silva IDCG, Lo Turco EG and Oliveira JSR
Article Type: Research article | First Published: January 25, 2020
The main barrier to the success of hematopoietic stem cell transplantation (HSCT) is acute graft versus host disease (aGVHD). The risk factors for aGVHD are mainly clinical and can be improved if additional biological factors are incorporated, such as the metabolomic profile. It is known that the levels of various metabolites measured are important in the process of immunoregulation. In our population study we investigated the systemic metabolic profile for 26 (Group I) allotransplant recipients...
Prospective Study of Autologous Adipose Derived Stromal Vascular Fraction Containing Stem Cells for the Treatment of Knee Osteoarthritis
Mark Berman, MD, Elliot Lander, MD, Thomas Grogan, MD, Walter O'Brien, MD, Jonathan Braslow, MD, Shawntae Dowell and Sean Berman, MS
Article Type: Original Article | First Published: November 29, 2019
The management of osteoarthritis of the knee runs the spectrum of care from a variety of conservative treatments often culminating in total joint arthroplasty. We initiated a large prospective study to evaluate whether autologous adipose derived stromal vascular fraction (SVF - rich in stem cells) therapy is a safe and effective option. A patient funded prospective study of 2,586 patients from a network of physicians participated in an IRB approved study using autologous stromal vascular fractio...
Selection of Cells for Parkinson's Disease Cell-Therapy
Ashok Chakraborty and Anil Diwan
Article Type: Research Article | First Published: November 25, 2019
Availability of DOPA in situ and thereafter Dopamine (DA) is the main therapeutic approach to treat the Parkinson's Disease (PD). Human neural stem cells (hNSCs), a good source of DA synthesis and release, have long been demonstrated to be a promising candidate for treating PD. However limited cell sourcing and low-growth rate are major concern of its applicability. Different types of other stem cells or reprogrammed somatic cells, including induced pluripotent stem cells (iPSCs), holds tremendo...
Mesenchymal Stem Cell Based Therapy for Parkinson's Disease
Neveen A Salem
Article Type: Review Article | First Published: September 23, 2019
Parkinson's disease (PD) is a chronic, progressive, neurodegenerative disease with a multifactorial etiology, the predominant pathology of PD is the loss of dopaminergic cells in the substantia nigra. It is characterized by hallmark signs of bradykinesia, rigidity, tremor, and postural instability. Medical and pharmacological treatments for Parkinson's disease are limited to the symptomatic relief of patients, and has failed to prevent or slow down the process of neurodegeneration. Cell transpla...
Late Intrathecal Cell Therapy Increases Brain Glucose Metabolism and Improves the Long-Term Established Sequelae of Cerebral Hemorrhage
Sara Fernandez-Guinea, Mercedes Zurita, Estefania Garcia, Jorge Mucientes, Cristina Lopez, Cecilia Fernandez-Mateos and Jesus Vaquero
Article Type: Case Report | First Published: December 24, 2018
The possibility of an increase in brain glucose metabolism after intrathecal administrations of autologous mesenchymal stromal cells (MSCs) has been recently reported, suggesting a role in the treatment of neurological disorders. We describe here the utility of this type of cell therapy in chronic neurological sequelae due to cerebral hemorrhage. A 49-year-old female suffered from 2009 severe neurological sequelae after spontaneous intracerebral hemorrhage open to ventricle in the left frontopar...
Mechanical Stimulation Increases the Proliferation and Differentiation Potential of Human Adipose-Derived Stromal Cells
Susanne Jung, Lauren Bohner, Kathrin Spindler, Marcel Hanisch, Johannes Kleinheinz and Sonja Sielker
Article Type: Original Article | First Published: December 05, 2018
Dynamic conditions stimulate the bone remodeling process by improving the nutrients transport and increasing the expression of osteogenic cells. The purpose of this study was to evaluate the effect of mechanical stimulation on the osteogenic differentiation of human adipose-derived stromal cells. Bone fracture defects require an interventional treatment which is dependent from the size of the injured site: Whereas smaller bone defects may be spontaneously solved and comprise minimal intervention...
Fluorescence-Activated on-Chip Cell Culture Sorting (O3CS): Smart Petri Dish
Pavel G Molchanov, Jorge E Moreno-Cuevas, Martin Hernandez, Maria Teresa Gonzalez-Garza, Charles Garcia, Edward William Hillhouse, Christine Lopez, Eva M Zsigmond, Rick Wetsel, John Mazzilli, Hong Zeng8, Jeewon Kim, Jieying Yang, Raquel Cuevas, Pavel M Bulai, Taras N Pitlik, Andrey A Denisov, Sergey N Cherenkevich, Alla Yu Molchanova, Elena N Golubeva, Victor A Strukov and Victor V Boksha
Article Type: Research Article | First Published: November 17, 2018
On-chip cell sorting is a promising technique for sorting stem cells in culture. On-chip cell sorting allows minimization of lab personnel involvement in cells processing, dramatically reducing the risk of cell culture contamination. We developed a fluorescence-activated On-Chip Cell Culture Sorting (O3CS) system, which combines a biocompatible semiconductor light addressable microarray (chip) and optical setup for chip addressing and cell culture observation. The optical setup has fluorescent a...
Direct and Simple Method for Mesenchymal Stem Cells Isolation, Culturing and Detection
Rafal H Abdullah, Nahi Yosef Yaseen, Shahlla M Saleh, Maeda H Mohamed and Ahmed Majeed Al-Shammari
Article Type: Research Article | First Published: October 05, 2018
Bone marrow mesenchymal stem cells (BM-MSCs) are the best adult stem cells that can be used for autologous regenerative medicine. These cells are easily manipulated for use in cell therapy. Therefore, rapid and simple method for culture MScs is needed. In this paper, we are showing simple and robust method for culturing the stem cells. The cells were flushed from the bone marrow of the thighbones of mice and cultured directly in tissue culture flask. Purification of BMSCs were based on the abili...