Short Communication Open Access

Hypercholesterolemic Diet: Its Effect on Colorectal Precancerous Lesions Induced by Dimethylhydrazine

Franciane da Silva Franca, Rodrigo Zeni Cora, Helena Cristina Ferreira Franz and Flavia Martinello
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: Objective: Evaluate the effects of dietary cholesterol, without the addition of cholic acid in the initial stage of carcinogenesis and on the bifidobacteria into the colon. Methods: Rats were grouped according to their diets and dimethylhydrazine treatment (DMH): standard diet (CN); CN + cholesterol 1% (CHOL); CN + DMH (CNDMH) and CN + cholesterol 1% + DMH (CHOLDMH). After eight weeks of treatment were analyzed weight gain, faecal pH, lipid profile, hepatic and faecal cholesterol, faecal bifidobacteria, cell proliferation and the pre-neoplastic colorectal lesions (aberrant crypt foci (ACF)).

PDF   | Full Text   DOI: 10.23937/2469-5807/1510040

Review Article Open Access

Japanese Crisis of HPV Vaccination

Masayuki Sekine, Risa Kudo, Sosuke Adachi, Manako Yamaguchi, Yutaka Ueda, Tomomi Takata, Akiko Morimoto, Yusuke Tanaka, Asami Yagi, Etsuko Miyagi and Takayuki Enomoto
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: Cervical cancer has been increasing in younger generation, especially in 20's and 30's, so it has been a serious problem that the disease leads to loss of fertility as an urgent issue in Japan. HPV vaccine was added to the national list of routine vaccinations for Japanese girls aged 12 to 16 years by law since April, 2013. Three months later, however, the Japanese Ministry of Health, Labour, and Welfare suspended recommendations for HPV vaccination after several reports of possible adverse events.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510039

Letter to the Editor Open Access

Myofibroblasts Role in Wound Healing of Εyelid Lesions

Trisevgeni Ntinioti, Georgia-Heleni Thomopoulou, Eugene Danas, Nikolaos G Kavantzas, Emmanuel Agapitos and Andreas C Lazaris
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: In injured tissues, the repair of the extracellular matrix is organized by activated myofibroblasts. The latter cells support harmonious mechanical tissue organization. At the end of the normal repair process, myofibroblasts disappear by apoptosis but in pathological situations, myofibroblasts likely remain leading to excessive scarring. The eyelids are movable folds of tissue the core of which is represented by a tarsal plate, composed of dense connective tissue, embedded in loose connective tissue. Τhe correlation between the healing process of surgically treated eyelid lesions and histological parameters is worth investigating.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510038

Case Report Open Access

Atypical Retinal Arterial Macroaneurysm

Hans Hoerauf, Philipp Steven and Nicolas Feltgen
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: A patient with an atypical retinal arterial macroaneurysm masked by a persistent vitreous haemorrhage mimicking a neoplasm is presented. During vitrectomy a pigmented peripapillary tumour located at large vessels with circinate lipid exsudates was detected. Examination of removed tissue revealed haemosiderin-loaded macrophages resulting in pigmentation. The histologic finding of phagocytosis of intraretinal blood remnants allowed to diagnose an atypical retinal arterial macroaneurysm.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510037

Review Article Open Access

BRAF V600E Immunohistochemistry in Cutaneous Langerhans Cell Histiocytosis: An Analysis of 20 Cases

Ryan C Romano, Wonwoo Shon, Sarah M Jenkins and Karen J Fritchie
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: Twenty dermatologic biopsy specimens from 14 patients (9 adults and 5 children) were retrieved. All cases were immunostained with BRAF mutation-specific antibody. Selected BRAF V600E IHC-positive cases were analyzed for BRAF V600E mutation by pyrosequencing. Clinical parameters were correlated with IHC status. BRAF V600E protein overexpression was identified in 15 of 20 (75%) cases (9/14 patients; 5 adults and 4 children). All pediatric patients with bone involvement were BRAF V600E IHC positive. 4/5 (80%) patients without BRAF V600E expression had CNS involvement (p = 0.02). The presence of BRAF V600E mutation was identified in 1/5 (20%) BRAF V600E IHC-positive cases.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510036

Case Report Open Access

Severe Complication of POPS: Mesh Malposition in the Bladder

Sabine Kersting, Dirk Kusche, Klaus-PeterJung and Eugen Berg
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: The authors present a case of a 68-years-old woman suffering of persistent urinary tract infections accompanied by intermittent fever. She had a history of pelvic organ prolapse suspension (POPS) for descending perineal syndrome with rectal prolapse, carried out elsewhere 15 months ago. Cystoscopy revealed a part of the prolene mesh that was incrusted along the fundus of the bladder to be the source of symptoms. This is probably the first case that reports a complication of POPS, which is a relative new surgical technique that gains more and more widespread use. At present no data exist about long-term results and complications. Although POPS is a minimally invasive procedure our case report shows that the risk of serious complications has to be taken in mind.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510035

Case Report and Literature Review Open Access

Opana-Induced Renal and Hepatic Injury: A Case Report and Review

Yahya M Kurdi, Sergey Brodsky and Martha Yearsley
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: A 34-year-old Caucasian female presented with headache, fatigue, mild abdominal pain, and dehydration. She admitted to intravenous abuse of "melted" Opana ER tablets (extended-release oral formulation of oxymorphone hydrochloride). Noted clinical signs included tachycardia, tachypnea, and trace peripheral edema. Abnormal laboratory tests included low creatinine clearance, hemoglobin, hematocrit, and platelet. Histological studies of hepatorenal biopsies showed evidence of severe active thrombotic microangiopathy (TMA) involving the arterioles and small arteries of the kidney and scattered portal and lobular granulomata and hepatic vascular changes involving the hepatic artery branches in some portal tract. To our knowledge of the published English literature, this is probably the first case report of Opana induced thrombotic microangiopathy (TMA) with evidence of histological involvement to both the kidney and the liver.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510034

Case Report and Literature Review Open Access

Anaplastic Large Cell Lymphoma as a Potential Diagnostic Challenge and Pitfall in Aspiration Biopsies of Thoracic Tumours

Justin Bateman, Celeste Morley, Bruce F. Burns and Shahid Islam
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: The patient is a 54 year-old male who presented in January 2015 with increasing dyspnea and weight loss over several months, eventually requiring intubation and admission to ICU for respiratory failure. He had a remote history of testicular seminoma and 'melanoma'. A trans-bronchial Wang needle aspirate biopsy of the right sub-carinal lymph node was preformed and showed sheets of large malignant cells with pleomorphic nuclei, multiple prominent nucleoli, and abundant cytoplasm. The tumour cells are positive for CD45, CD3, CD30 and ALK-1.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510033

Review Article Open Access

Epigenomic Explanations for the Uncertainty of Cancer Biomarkers

Shou-Tung Chen, Chia-Chen Hsu, Yu-Wei Leu and Shu-Huei Hsiao
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: Inheritable epigenetic modifications, like histone modifications and DNA methylation, were once considered somatically stable and tissue-specific, yet accumulating evidence suggests the contrary. Environmental encounters are transduced into the cell through signaling pathways and these signals are relayed to the nucleus and memorized as epigenetic marks on target genes. Signaling-specific epigenomic changes provide selectable outlines for further lineage determination during differentiation and serve as traceable biomarkers of specific signaling pathways during transformation. Current "big data" interrogation employs signal-specific reduction methods to identify epigenetically modified genes as possible signal targets.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510032

Mini Review Open Access

BerEP4 and AE1/3 are Reliable Markers of Epithelial Content for Biomarker Discovery Using Reverse Phase Protein Arrays (RPPA)

Ola H Negm, James C Hassall, Wakkas Fadhil, Alain Pitiot, Patrick J Tighe, Cedric Marchessoux and Mohammad Ilyas
Int J Pathol Clin Res Volume 2, Issue 2

Abstract: Biomarker studies are notorious for their high failure rate. Despite huge investment by both academia and industry, very few biomarkers make the transition from interesting laboratory data to tests with clinical utility. The reasons for this are manifold. Some biomarkers are "true" biomarkers inasmuch as they are robust and reproducible but the information they provide does not help to improve patient management beyond that informed by traditional clinical parameters.

PDF   | Full Text   DOI: 10.23937/2469-5807/1510031

Volume 2
Issue 2